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MyBiosource Biotechnology elisa kit ahr elisa kit, catalog no. mbs702604
Elisa Kit Ahr Elisa Kit, Catalog No. Mbs702604, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ahr+elisa+kit/sandwich+elisa++enzyme+linked+immunosorbent+assay+/pm29802768-47-8-16
Average 90 stars, based on 1 article reviews
elisa kit ahr elisa kit, catalog no. mbs702604 - by Bioz Stars, 2026-10
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Enzyme-linked Immunosorbent Assay:

Article Title: Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense AhR ELISA kit (MyBioSource, San Diego, USA) according to the manufacturer’s protocols. ..



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Expression of the aryl hydrocarbon <t>receptor</t> <t>(AhR)</t> in placenta-derived mesenchymal stem cells (PD-MSCs). (a) PD-MSCs were cultured with BP for 7 days, and mRNA was extracted. Expression of AhR was evaluated by RT-PCR. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) Phase contrast images were obtained before and after treatment of 1 μ M BP for 7 days (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. (c) Cell lysates were collected after PD-MSCs were cultured with 1 μ M BP for 7 days. AhR concentration in cultured PD-MSCs was confirmed by <t>ELISA.</t> The data are expressed as the mean ± SD of three independent experiments. ∗ P < 0.05.
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Expression of the aryl hydrocarbon <t>receptor</t> <t>(AhR)</t> in placenta-derived mesenchymal stem cells (PD-MSCs). (a) PD-MSCs were cultured with BP for 7 days, and mRNA was extracted. Expression of AhR was evaluated by RT-PCR. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) Phase contrast images were obtained before and after treatment of 1 μ M BP for 7 days (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. (c) Cell lysates were collected after PD-MSCs were cultured with 1 μ M BP for 7 days. AhR concentration in cultured PD-MSCs was confirmed by <t>ELISA.</t> The data are expressed as the mean ± SD of three independent experiments. ∗ P < 0.05.
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Expression of the aryl hydrocarbon receptor (AhR) in placenta-derived mesenchymal stem cells (PD-MSCs). (a) PD-MSCs were cultured with BP for 7 days, and mRNA was extracted. Expression of AhR was evaluated by RT-PCR. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) Phase contrast images were obtained before and after treatment of 1 μ M BP for 7 days (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. (c) Cell lysates were collected after PD-MSCs were cultured with 1 μ M BP for 7 days. AhR concentration in cultured PD-MSCs was confirmed by ELISA. The data are expressed as the mean ± SD of three independent experiments. ∗ P < 0.05.

Journal: Stem Cells International

Article Title: Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor

doi: 10.1155/2019/7414015

Figure Lengend Snippet: Expression of the aryl hydrocarbon receptor (AhR) in placenta-derived mesenchymal stem cells (PD-MSCs). (a) PD-MSCs were cultured with BP for 7 days, and mRNA was extracted. Expression of AhR was evaluated by RT-PCR. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) Phase contrast images were obtained before and after treatment of 1 μ M BP for 7 days (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. (c) Cell lysates were collected after PD-MSCs were cultured with 1 μ M BP for 7 days. AhR concentration in cultured PD-MSCs was confirmed by ELISA. The data are expressed as the mean ± SD of three independent experiments. ∗ P < 0.05.

Article Snippet: Cell lysates were assayed immediately by a human AhR ELISA kit (MyBioSource, San Diego, USA) according to the manufacturer's protocols.

Techniques: Expressing, Derivative Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Negative Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

StemRegenin 1 (SR1) maintains placenta-derived mesenchymal stem cell (PD-MSC) activity and decreases BP-induced apoptosis. (a) The expression levels of AhR were determined by RT-PCR after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) The concentration of AhR was analyzed by ELISA after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (c) The proliferative activity of PD-MSCs was measured by EZ-CYTOX after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (d) Culture of PD-MSCs with 1 μ M SR1 prevents cellular senescence (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. The number of β -gal positive cells was enumerated after 4 weeks. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (e) Confocal microscopy images were collected after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. One representative of 3 independent experiments is shown (scale bar, 20 μ m; magnification, 200x). (f) RT-PCR analysis revealed diminished expression of caspase-3 in treatment of 1 μ M BP with 1 μ M SR1. The data are expressed as the mean ± SD of three independent experiments.

Journal: Stem Cells International

Article Title: Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor

doi: 10.1155/2019/7414015

Figure Lengend Snippet: StemRegenin 1 (SR1) maintains placenta-derived mesenchymal stem cell (PD-MSC) activity and decreases BP-induced apoptosis. (a) The expression levels of AhR were determined by RT-PCR after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) The concentration of AhR was analyzed by ELISA after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (c) The proliferative activity of PD-MSCs was measured by EZ-CYTOX after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (d) Culture of PD-MSCs with 1 μ M SR1 prevents cellular senescence (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. The number of β -gal positive cells was enumerated after 4 weeks. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (e) Confocal microscopy images were collected after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. One representative of 3 independent experiments is shown (scale bar, 20 μ m; magnification, 200x). (f) RT-PCR analysis revealed diminished expression of caspase-3 in treatment of 1 μ M BP with 1 μ M SR1. The data are expressed as the mean ± SD of three independent experiments.

Article Snippet: Cell lysates were assayed immediately by a human AhR ELISA kit (MyBioSource, San Diego, USA) according to the manufacturer's protocols.

Techniques: Derivative Assay, Activity Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Confocal Microscopy