Journal: Stem Cells International
Article Title: Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor
doi: 10.1155/2019/7414015
Figure Lengend Snippet: StemRegenin 1 (SR1) maintains placenta-derived mesenchymal stem cell (PD-MSC) activity and decreases BP-induced apoptosis. (a) The expression levels of AhR were determined by RT-PCR after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. One representative of 3 independent experiments is shown. Expression levels relative to GAPDH are shown. (−) indicates a negative control using water alone. (b) The concentration of AhR was analyzed by ELISA after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (c) The proliferative activity of PD-MSCs was measured by EZ-CYTOX after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (d) Culture of PD-MSCs with 1 μ M SR1 prevents cellular senescence (scale bar, 200 μ m; magnification, 100x). One representative of 3 independent experiments is shown. The number of β -gal positive cells was enumerated after 4 weeks. The data are expressed as the mean ± SD of three independent experiments. ∗∗ P < 0.01. (e) Confocal microscopy images were collected after treatment of 1 μ M BP and 1 μ M BP with 1 μ M SR1 for 7 days, respectively. One representative of 3 independent experiments is shown (scale bar, 20 μ m; magnification, 200x). (f) RT-PCR analysis revealed diminished expression of caspase-3 in treatment of 1 μ M BP with 1 μ M SR1. The data are expressed as the mean ± SD of three independent experiments.
Article Snippet: Cell lysates were assayed immediately by a human AhR ELISA kit (MyBioSource, San Diego, USA) according to the manufacturer's protocols.
Techniques: Derivative Assay, Activity Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Confocal Microscopy